Microsporidia Stain, Varies

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Detecting microsporidiosis beyond the intestines, affects organs like lungs and skin, particularly in those with weakened immune systems.

Microsporidia Stain, Varies

 

OVERVIEW

The Trichrome-Blue Stain (Ryan Modification), pending availability confirmation, is used for Microsporidia Stain to detect organisms such as Encephalitozoon and Enterocytozoon. Specimens needed include duodenal aspirates, respiratory secretions, eye fluids, fresh tissues, and gallbladder or bile aspirates. Each specimen type has specific container and volume requirements, with stability guidelines for preservation methods like ECOFIX, formalin, refrigeration, or freezing.


 

METHOD NAME

Trichrome-Blue Stain (Ryan Modification)

 

AVAILABILITY

TBC

 

REPORTING NAME

Microsporidia Stain
 
 

ALIASES

Encephalitozoon

Entercytozoon

Nosema

Pleistophora

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SPECIMEN TYPE

Varies

 

SHIPPING INSTRUCTIONS

TBC

 

SPECIMEN REQUIRED

Submit only 1 of the following specimens:

 

Specimen Type: Duodenal aspirate (small intestinal aspirate, jejunal aspirate, small bowel aspirate)

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Additional Information: ECOFIX and 10% formalin are acceptable preservatives.

Specimen Stability Information: Preserved Ambient (preferred) <10 days/Refrigerated <3 days

 

Specimen Type: Respiratory secretions (bronchoalveolar lavage [BAL], sputum, bronchial wash, pleural fluid)

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Specimen Stability Information: Refrigerated <3 days (preferred)/Frozen <10 days

 

Specimen Type: Eye (vitreous fluid, corneal scraping, ocular fluid)

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Specimen Stability: Refrigerated <3 days

 

Specimen Type: Fresh tissue (lung, eye, bladder, rectal, intestinal, colon, skin, muscle, kidney)

Container/Tube: Sterile container

Specimen Volume: 3-mm biopsy in 0.1-mL sterile saline

Specimen Stability: Refrigerated <3 days

 

Specimen Type: Gallbladder aspirate/Bile aspirate

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Specimen Stability: Refrigerated <3 days/Frozen <10 days

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